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角膜组织工程: 人角膜间质神经相互作用的体外模型
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Developmental Biology
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JoVE Journal Developmental Biology
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
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角膜组织工程: 人角膜间质神经相互作用的体外模型

DOI:
10.3791/56308-v

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07:35 min

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January 24, 2018

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, , , ,
1Department of Cell Biology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology/Dean McGee Eye Institute,University of Oklahoma Health Sciences Center, 3Department of Physiology, Harold Hamm Diabetes Center,University of Oklahoma Health Sciences Center

Chapters

  • 00:05Title
  • 01:17Isolation of Primary Cells
  • 04:04Primary HCF Culture and 3D Construct Assembly
  • 05:00Cell Differentiation and Co-culture
  • 06:17Results: Transmission Electron Microscopy of the 3D Self-assembled Construct
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

本议定书描述了一个新的三维的体外模型, 其中角膜基质细胞和分化的神经细胞被培养在一起, 以协助检查和理解两种细胞的相互作用。

Tags

Stromal-nerve InteractionsHuman CorneaCorneal DiseasesCorneal DystrophiesExtracellular MatrixCell-cell InteractionsDrug ScreeningCorneal Nerve DamageCorneal Nerve Regeneration

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