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Hornhindevæv Engineering: En In Vitro Model af den menneskelige hornhinden stromale-nerve interaktioner
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Developmental Biology
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JoVE Journal Developmental Biology
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Hornhindevæv Engineering: En In Vitro Model af den menneskelige hornhinden stromale-nerve interaktioner

DOI:
10.3791/56308-v

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07:35 min

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January 24, 2018

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, , , ,
1Department of Cell Biology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology/Dean McGee Eye Institute,University of Oklahoma Health Sciences Center, 3Department of Physiology, Harold Hamm Diabetes Center,University of Oklahoma Health Sciences Center

Chapters

  • 00:05Title
  • 01:17Isolation of Primary Cells
  • 04:04Primary HCF Culture and 3D Construct Assembly
  • 05:00Cell Differentiation and Co-culture
  • 06:17Results: Transmission Electron Microscopy of the 3D Self-assembled Construct
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en ny tre-dimensionelle in vitro- model, hvor hornhinde stromale celler og differentieret neuronale celler dyrkes sammen til at bistå med gennemgang og forståelse af samspillet mellem to celletyper.

Tags

Stromal-nerve InteractionsHuman CorneaCorneal DiseasesCorneal DystrophiesExtracellular MatrixCell-cell InteractionsDrug ScreeningCorneal Nerve DamageCorneal Nerve Regeneration

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