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JoVE Journal Neuroscience
Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
 

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Article DOI: 10.3791/56425-v • 11:12 min • October 4th, 2017
October 4th, 2017 •
1, 1, 2, 3
1Automation & Logistics Management, Screening Sciences & Novel Assay Technologies, Institut Pasteur Korea, 2School of Pharmacy, Sungkyunkwan University, 3Technology Development Platform, Institut Pasteur Korea

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Summary
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We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.

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Keywords: Assay Development High Content Quantification Sod1 Mutant Protein Protein Aggregate Formation Living Cells Neurodegenerative Disorders ALS Lentivirus Production HEK-293 T-cells Transfection Cell Transduction
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