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测定化学物质对线虫线虫生长和繁殖的影响

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Medicine

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JoVE Journal Medicine

Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans

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测定化学物质对线虫线虫生长和繁殖的影响

DOI:

10.3791/56437-v

•

08:12 min

•

October 05, 2017

•

So Young Lee, Kyungsu Kang

¹Systems Biotechnology Research Center,Korea Institute of Science and Technology

Chapters

00:05Title
00:55Preparation of NGM Plates Containing Either 1% DMSO or 750 μM Etoposide
03:44Measurement of C. elegans Growth
05:26C. elegans Egg Laying Assay
06:18Results: C. elegans Growth and Reproduction with Etoposide Treatment
07:41Conclusion

Summary

Automatic Translation

一个基本的协议, 以评估化学品的毒性在模型动物,线虫线虫, 描述。该方法对药品的开发和各种环境污染物的风险评估都具有很好的实用价值。

Tags

Caenorhabditis Elegans Chemical Effects Growth Reproduction Toxicology Etoposide DMSO NGM Medium E. Coli OP50 Age-synchronized Eggs

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