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En protokol til Decellularizing musen Cochleae for indre øre vævsmanipulering
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Bioengineering
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JoVE Journal Bioengineering
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

En protokol til Decellularizing musen Cochleae for indre øre vævsmanipulering

DOI:
10.3791/56523-v

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09:53 min

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January 01, 2018

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, , , ,
1Department of Otolaryngology,University of Kansas Medical Center, 2Stephenson School of Biomedical Engineering,University of Oklahoma, 3Department of Plastic Surgery,University of Kansas Medical Center

Chapters

  • 00:05Title
  • 01:05Temporal Bone Harvest and Cochlea Isolation
  • 03:07Cochlear Processing
  • 05:11Infusion of hWJCs into Decellularized Cochleae
  • 06:58Results: Cell Culture on Decellularized, Decalcified Mouse Cochleae
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er at påvise en effektiv metode til at decellularize og afkalke mus cochleae for udnyttelse som stilladser for væv ingeniørmæssige anvendelser.

Tags

DecellularizationDecalcificationMouse CochleaExtracellular MatrixStem Cell DifferentiationSensory EpitheliumTemporal BoneBony LabyrinthStapesOval WindowRound WindowPerilymph1% SDS SolutionPBSAntibiotic-antimycoticPenicillin-streptomycin

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