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渗透胁迫对分泌性囊泡和胞吐作用的监测作用
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Neuroscience
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JoVE Journal Neuroscience
Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
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渗透胁迫对分泌性囊泡和胞吐作用的监测作用

DOI:
10.3791/56537-v

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08:08 min

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February 19, 2018

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, , ,
1Department of Chemistry and Chemical Engineering,Chalmers University of Technology, 2Department of Chemistry and Molecular Biology,University of Gothenburg

Chapters

  • 00:04Title
  • 00:50Single Cell Exocytosis Amperometry Experiments
  • 04:00Intracellular Electrochemical Cytometry
  • 06:47Results: The Effect of Osmotic Pressure on Exocytosis Activity
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

渗透胁迫影响胞吐作用和在这个过程中释放的神经递质的数量。我们演示了如何结合电化学方法和透射电子显微术来研究细胞外渗透压对胞吐作用活性、囊泡量子大小和释放神经递质的影响。在胞吐作用期间。

Tags

Keywords: – Osmotic Stress – Secretory Vesicles – Exocytosis – Amperometric Recording – Carbon Fiber Microelectrode – Cyclic Voltammetry – Chromaffin Cells – Patch Clamp – Faraday Cage – Microscope Heating Stage

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