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Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
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Chapters
- 00:05Title
- 01:01Establishing 3D Co-cultures with Indirect Interaction
- 02:133D Co-cultures with Direct Interaction
- 06:14Characterization of the Effects of Supernatants from Primary BrC Cells on Acini Formation and Acini Structure
- 08:28Results: Communication between Monocytes and Primary BrC Cells in an ECME-3D System
- 10:01Conclusion
Here, we describe a three-dimensional culture method to analyze the morphology of primary breast cancer cells, as well as to study their direct/indirect interactions with monocytes and the outcomes such as collagen degradation, immune cell recruitment, cell invasion, and promotion of cancer-related inflammation.
