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基于细胞外基质提取物 (ECME) 的三维系统中单核细胞与原代乳癌细胞的通讯分析
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Cancer Research
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JoVE Journal Cancer Research
Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
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基于细胞外基质提取物 (ECME) 的三维系统中单核细胞与原代乳癌细胞的通讯分析

DOI:
10.3791/56589-v

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10:55 min

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January 08, 2018

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, ,
1Unidad de Investigación en Virología y Cáncer,Hospital Infantil de México Federico Gómez, 2Programa de Doctorado en Ciencias Biomédicas, Facultad de Medicina,Universidad Nacional Autónoma de México

Chapters

  • 00:05Title
  • 01:01Establishing 3D Co-cultures with Indirect Interaction
  • 02:133D Co-cultures with Direct Interaction
  • 06:14Characterization of the Effects of Supernatants from Primary BrC Cells on Acini Formation and Acini Structure
  • 08:28Results: Communication between Monocytes and Primary BrC Cells in an ECME-3D System
  • 10:01Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一个三维的培养方法来分析原发性乳腺癌细胞的形态学, 以及研究他们的直接/间接相互作用的单核和结果, 如胶原降解, 免疫细胞招募, 细胞侵袭和促进癌症相关的炎症。

Tags

Keywords: 3D Co-cultureMonocyte-breast Cancer Cell CommunicationTumor MicroenvironmentExtracellular Matrix Extract (ECME)U937 CellsTHP-1 CellsPrimary Breast Cancer CellsCell LabelingFluorescent DyesTrypsinization

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