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Cella singola quantificazione dei tassi di degradazione delle proteine mediante microscopia a fluorescenza time-lapse in coltura delle cellule aderenti
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Chapters
- 00:00Title
- 01:42Cell seeding and pulse labeling of the SNAP-tag
- 04:15Time-lapse microscopy
- 05:23Image processing and analysis
- 07:55Results
- 10:13Conclusions
Tags
Keywords:
Protein DegradationSingle-cellTime-lapse Fluorescence MicroscopyAdherent Cell CultureSNAP-tagProtein Half-lifeFluorescence ImagingProtein TurnoverHeterogeneityEndogenous Protein TaggingOverexpressionDNA Repair EnzymeBenzylguanineFluorescent DyeExponential DecayEmbryonic Stem CellsMonolayerCell-cell InteractionsN-cadherin
