Journal
/
/
En nevrale nettverk-basert identifikasjon av Developmentally kompetent eller inkompetent musen Fully-Grown Oocytes
JoVE Journal
Developmental Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes
DOI:

10:04 min

March 03, 2018

, , , , , , ,

Chapters

  • 00:00Title
  • 00:14Introduction
  • 02:03Cumulus Oocyte Complex isolation
  • 03:25Oocyte classification with fluorochrome Hoechst 33342
  • 04:56Neural network-based oocyte classification
  • 06:12Results
  • 09:38Conclusion

Summary

Automatic Translation

Her presenterer vi en protokoll for ikke-invasiv vurdering av oocyte utviklingsmessige kompetanse utført under deres i vitro modning fra germinal vesicle på metaphase II scenen. Denne metoden kombinerer tidsinnstilt bildebehandling med partikkel bilde velocimetry (PIV) og nettverk analyser.

Related Videos

Read Article