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Journal
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Biochemistry
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一种用于小数目造血干细胞的西方印迹协议
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
一种用于小数目造血干细胞的西方印迹协议
DOI:
10.3791/56855-v
•
08:45 min
•
August 22, 2018
•
Xiongwei Cai
2,3
,
Yi Zheng
,
Nancy A. Speck
1
Division of Experimental Hematology
,
Cincinnati Children’s Hospital Medical Center
,
2
Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute
,
Perelman School of Medicine, University of Pennsylvania
,
3
Department of Obstetrics and Gynecology, Southwest Hospital
,
Third Military Medical University
Chapters
00:04
Title
00:48
Sample Preparation
04:20
Electrophoresis, Transfer, and Block
05:30
Antibody Labeling and Detection
06:09
Results: Use of Optimized Western Blotting Technique to Analyze Small Numbers of Hematopoietic Stem and Progenitor Cells
07:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
通过对500只造血干细胞或祖细胞进行分析, 优化了标准的西方印迹协议。优化包括仔细处理细胞样本, 限制管道之间的传输, 并直接株溶藻 Laemmli 样本缓冲区中的细胞。
Tags
Keywords: Western Blotting
Hematopoietic Stem Cells
Protein Analysis
Cell Sorting
Protein Quantification
Low Cell Number
Cell Lysis
Sample Preparation
Centrifugation
Phosphatase Inhibitors
Protease Inhibitors
Laemmli Buffer
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