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一种用于小数目造血干细胞的西方印迹协议
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
DOI:

08:45 min

August 22, 2018

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Chapters

  • 00:04Title
  • 00:48Sample Preparation
  • 04:20Electrophoresis, Transfer, and Block
  • 05:30Antibody Labeling and Detection
  • 06:09Results: Use of Optimized Western Blotting Technique to Analyze Small Numbers of Hematopoietic Stem and Progenitor Cells
  • 07:56Conclusion

Summary

Automatic Translation

通过对500只造血干细胞或祖细胞进行分析, 优化了标准的西方印迹协议。优化包括仔细处理细胞样本, 限制管道之间的传输, 并直接株溶藻 Laemmli 样本缓冲区中的细胞。

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