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En Western Blotting protokoll för litet numrerar av hematopoetiska stamceller
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1Division of Experimental Hematology,Cincinnati Children’s Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute,Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital,Third Military Medical University
Chapters
- 00:04Title
- 00:48Sample Preparation
- 04:20Electrophoresis, Transfer, and Block
- 05:30Antibody Labeling and Detection
- 06:09Results: Use of Optimized Western Blotting Technique to Analyze Small Numbers of Hematopoietic Stem and Progenitor Cells
- 07:56Conclusion
Standard Western blotting protokollet var optimerad för att analysera så få som 500 hematopoetiska stamceller eller stamceller. Optimering innebär noggrann hantering av cell provet, begränsa överföringar mellan rören och direkt lyseringslösning cellerna i Laemmli prov buffert.
