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Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

DOI:
10.3791/56960-v

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08:04 min

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December 18, 2017

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, , , ,
1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

Chapters

  • 00:05Title
  • 00:45Sterile Microinjection
  • 04:07Pharmacological Treatment of HIOs
  • 05:13Results: Epithelial Barrier Permeability in HIOs is Affected by Bacterial Toxin
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the measurement of epithelial barrier permeability in real-time following pharmacologic treatment in human intestinal organoids using fluorescent microscopy and live cell microscopy.

Tags

Epithelial Barrier PermeabilityHuman Intestinal OrganoidsReal-time MeasurementFITC-dextranMicroinjectionGastroenterologyToxinsBacterial ProductsPharmacologic TreatmentsVisualizationQuantitative Measure

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