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Journal
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Developmental Biology
/
人肠道 Organoids 上皮屏障通透性的实时测量
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
Please note that all translations are automatically generated.
Click here for the English version.
人肠道 Organoids 上皮屏障通透性的实时测量
DOI:
10.3791/56960-v
•
08:04 min
•
December 18, 2017
•
David R. Hill
,
Sha Huang
,
Yu-Hwai Tsai
,
Jason R. Spence
2
,
Vincent B. Young
3
1
Department of Internal Medicine, Division of Gastroenterology
,
University of Michigan
,
2
Department of Cell and Developmental Biology
,
University of Michigan
,
3
Department of Internal Medicine, Division of Infectious Disease
,
University of Michigan
Chapters
00:05
Title
00:45
Sterile Microinjection
04:07
Pharmacological Treatment of HIOs
05:13
Results: Epithelial Barrier Permeability in HIOs is Affected by Bacterial Toxin
06:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了在人体肠道 organoids 中采用荧光显微镜和活细胞显微镜进行药物治疗后 real-time 上皮屏障通透性的测量。
Tags
Epithelial Barrier Permeability
Human Intestinal Organoids
Real-time Measurement
FITC-dextran
Microinjection
Gastroenterology
Toxins
Bacterial Products
Pharmacologic Treatments
Visualization
Quantitative Measure
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