/

/

一种基于液滴的单链 dna 放大术和微球 pcr 扩增法

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Please note that all translations are automatically generated. Click here for the English version.

一种基于液滴的单链 dna 放大术和微球 pcr 扩增法

DOI:

10.3791/57703-v

•

11:40 min

•

November 14, 2018

•

Se Hee Lee*¹, Ho Won Lee*², Da Som Kim, Hyuck Gi Kwon, Jong Hyun Lee, Yang-Hoon Kim, Ok Chan Jeong³, Ji-Young Ahn

¹College of Natural Sciences,Chungbuk National University, ²Department of Biomedical Engineering,Inje University, ³Institute of Digital Anti-Aging Healthcare,Inje University

Chapters

00:04Title
01:35Fabrication of a PDMS Microfluidic Platform
03:16Production of Polyacrylamide Oligo-Microspheres
06:06Performing DNA Hybridization on the Surface of Polyacrylamide Oligomicrosphere
07:06Microsphere-PCR for Amplifying ssDNA
08:08Confocal Microscopy Acquisition
08:38“Results: Fabrication of Droplet-base Microfluidic Platform and Application of Microspheres for Microsphere-PCR Amplification”
10:33Conclusion

Summary

Automatic Translation

本工作为液滴基微流体平台的制备和聚丙烯酰胺微球在微球 pcr 扩增中的应用提供了一种方法。微球 pcr 方法可以在不分离双链 dna 的情况下获得单链 dna 扩增器。

Tags

Keywords: Microfluidic Microsphere PCR Single-stranded DNA MEMS PDMS Flow-focusing Hydrophilic Corona Treater Acrydite Acrylamide

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

一个单粒子为基础的微流控液力陷阱

研究蛋白质折叠的微流体混合器

一种微流体装置研究多个不同的菌株

皮肤纹身作为一种新的方法DNA疫苗交货

预设计尺寸和形状的聚合物纤维和生物贿赂纤维的微流体制造

微流体为基础的电化学生物芯片的无标记的DNA杂交分析

微流体芯片ICPMS进样

高通量单细胞的分离和文化的微流体平台

多级可变高度的光刻多层带瓣微流体装置

与复用电子检测的粒子的空间跟踪微流体平台

Read Article