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Bir damlacık dayalı mikrosıvısal yaklaşım ve Microsphere-PCR güçlendirme tek iplikçikli DNA'Amplicons için
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
DOI:

11:40 min

November 14, 2018

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Chapters

  • 00:04Title
  • 01:35Fabrication of a PDMS Microfluidic Platform
  • 03:16Production of Polyacrylamide Oligo-Microspheres
  • 06:06Performing DNA Hybridization on the Surface of Polyacrylamide Oligomicrosphere
  • 07:06Microsphere-PCR for Amplifying ssDNA
  • 08:08Confocal Microscopy Acquisition
  • 08:38“Results: Fabrication of Droplet-base Microfluidic Platform and Application of Microspheres for Microsphere-PCR Amplification”
  • 10:33Conclusion

Summary

Automatic Translation

Bu eser mikrosıvısal damlacık tabanlı platformlar imalatı ve polyacrylamide mikroküreler microsphere-PCR güçlendirme için uygulanması için bir yöntem sağlar. Microsphere-PCR yöntemi, Çift iplikçikli DNA ayıran olmadan tek iplikçikli DNA amplicons elde olanak verir.

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