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果蝇蛹中炎症细胞动力学的长期体内追踪
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae
DOI:

09:26 min

June 14, 2018

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Chapters

  • 00:04Title
  • 00:51Preparing the Pupae
  • 03:12Laser-induced Wounding of Drosophila Pupal Wings
  • 04:41In Vivo Time-lapse Confocal Imaging
  • 06:52Results: Sterile Wounding Activates a Robust Inflammatory Response in the Drosophila Pupal Wing
  • 08:28Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个实时成像伤口修复的协议和相关的炎症反应在高时空分辨率的体内。该方法利用果蝇发育的蛹阶段, 实现对特定细胞种群的长期成像和跟踪, 并与有效的 rna 干扰介导的基因失活相兼容。

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