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Journal
/
Immunology and Infection
/
果蝇
蛹中炎症细胞动力学的长期
体内
追踪
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Long-term
In Vivo
Tracking of Inflammatory Cell Dynamics Within
Drosophila
Pupae
Please note that all translations are automatically generated.
Click here for the English version.
果蝇
蛹中炎症细胞动力学的长期
体内
追踪
DOI:
10.3791/57871-v
•
09:26 min
•
June 14, 2018
•
Helen Weavers
2
,
Anna Franz
,
Will Wood
,
Paul Martin
4
1
School of Biochemistry, Biomedical Sciences
,
University of Bristol
,
2
School of Cellular and Molecular Medicine, Biomedical Sciences
,
University of Bristol
,
3
MRC Centre for Inflammation Research
,
University of Edinburgh, Queens Medical Research Institute
,
4
School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences
,
University of Bristol
Chapters
00:04
Title
00:51
Preparing the Pupae
03:12
Laser-induced Wounding of
Drosophila
Pupal Wings
04:41
In Vivo
Time-lapse Confocal Imaging
06:52
Results: Sterile Wounding Activates a Robust Inflammatory Response in the
Drosophila
Pupal Wing
08:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个实时成像伤口修复的协议和相关的炎症反应在高时空分辨率
的体内
。该方法利用
果蝇
发育的蛹阶段, 实现对特定细胞种群的长期成像和跟踪, 并与有效的 rna 干扰介导的基因失活相兼容。
Tags
In Vivo Tracking
Inflammatory Cell Dynamics
Drosophila Pupae
Live Imaging
Wound Healing
Innate Immunity
RNAi
Gene Inactivation
Pupal Stage
Hemocyte
Pupae Collection
Pupal Casing
Dissection
Mounting
Heptane Glue
Article
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