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Indringende cel mechanica met kraal-vrije optisch pincet in de Drosophila Embryo
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo
DOI:

08:23 min

November 02, 2018

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Chapters

  • 00:04Title
  • 00:49Preparing the Optical Tweezers
  • 02:01Interfacing the Instrument
  • 02:52Calibration of the Optical Trap
  • 03:45Position Interpolation with Image Analysis
  • 04:30Mounting Drosophila Embryos
  • 05:12Trapping Experiment In Vivo
  • 06:06Mechanical Measurements
  • 06:44Results: Interface Deflection Imposed by Sinusoidal Movement
  • 07:50Conclusion

Summary

Automatic Translation

Presenteren we een installatie van optisch pincet gekoppeld aan een lichte blad Microscoop, en de uitvoering daarvan om te sonderen cel mechanica zonder kralen in de Drosophila embryo.

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