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Sondando a célula mecânica com pinça óptica de grânulo-livre no embrião da drosófila
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo
DOI:

08:23 min

November 02, 2018

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Chapters

  • 00:04Title
  • 00:49Preparing the Optical Tweezers
  • 02:01Interfacing the Instrument
  • 02:52Calibration of the Optical Trap
  • 03:45Position Interpolation with Image Analysis
  • 04:30Mounting Drosophila Embryos
  • 05:12Trapping Experiment In Vivo
  • 06:06Mechanical Measurements
  • 06:44Results: Interface Deflection Imposed by Sinusoidal Movement
  • 07:50Conclusion

Summary

Automatic Translation

Apresentamos uma instalação da pinça óptica acoplada a um microscópio de folha de luz e a sua aplicação a sonda mecânica de células sem grânulos no embrião da drosófila .

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