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Chapters
- 00:04Title
- 00:49Alkaline Hydrolysis of Genomic DNA
- 02:36Strand specific Southern Blot: Transfer
- 04:37Strand-specific Southern Blot: Hybridization
- 06:48Results: Assessment of Ribonucleotide Incorporation into DNA
- 08:08Conclusion
Ribonucleotides er blandt de mest rigelige ikke-kanoniske nukleotider indarbejdet i genomet i eukaryote nukleare DNA-replikation. Hvis ikke ordentligt fjernes, kan ribonucleotides forårsage DNA-skader og mutagenese. Vi præsenterer her, to eksperimentelle metoder, der bruges til at vurdere overfloden af ribonucleotide indarbejdelse i DNA og dets mutagene virkninger.
Tags
Keywords:
Ribonucleotide IncorporationStrand-specific DetectionYeast GenomeRibonucleotide-induced MutagenesisAlkaline Gel ElectrophoresisSouthern BlottingGenomic DNAPotassium HydroxideAlkaline DNA Loading BufferAgarose GelSodium HydroxideEDTADNA Size MarkerNeutralization BufferSYBR Gold StainUV TransilluminatorAlkaline Transfer BufferNylon MembraneCapillary Action
