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雌激素原代牛颗粒细胞的组织培养模型
JoVE Journal
Biology
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JoVE Journal
Biology
A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
Please note that all translations are automatically generated.
Click here for the English version.
雌激素原代牛颗粒细胞的组织培养模型
DOI:
10.3791/58208-v
•
05:36 min
•
September 06, 2018
•
Anja Baufeld
,
Jens Vanselow
1
Institute of Reproductive Biology
,
Leibniz Institute for Farm Animal Biology (FBN)
Chapters
00:04
Title
00:34
Isolation of Bovine Granulosa Cells (GC)
01:37
Cryopreservation of Cells
02:56
Cell Culture
03:53
Results: Analysis of Culture Bovine Granulosa Cells Under Various Conditions
04:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了牛颗粒细胞在无血清条件下的长期培养模型。该模型允许研究者研究不同电镀密度对雌激素产生的牛颗粒细胞特性的影响。
Tags
Estrogen-producing
Primary Bovine Granulosa Cells
Cryopreservation
Cell Isolation
Follicular Fluid
Granulosa Cells
Cell Culture
RNA
DNA
Protein Analysis
Cell Freezing
Cell Thawing
Alpha MEM
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