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Biology
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細胞のエストロゲン産生主ウシ顆粒の組織培養モデル
JoVE Journal
Biology
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JoVE Journal
Biology
A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
Please note that all translations are automatically generated.
Click here for the English version.
細胞のエストロゲン産生主ウシ顆粒の組織培養モデル
DOI:
10.3791/58208-v
•
05:36 min
•
September 06, 2018
•
Anja Baufeld
,
Jens Vanselow
1
Institute of Reproductive Biology
,
Leibniz Institute for Farm Animal Biology (FBN)
Chapters
00:04
Title
00:34
Isolation of Bovine Granulosa Cells (GC)
01:37
Cryopreservation of Cells
02:56
Cell Culture
03:53
Results: Analysis of Culture Bovine Granulosa Cells Under Various Conditions
04:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
無血清条件下でウシ顆粒膜細胞の長期培養モデルを説明します。このモデルにより、エストロゲン産生特性の異なるめっき密度として多様な因子と条件の効果を研究に研究をウシ顆粒膜細胞。
Tags
Estrogen-producing
Primary Bovine Granulosa Cells
Cryopreservation
Cell Isolation
Follicular Fluid
Granulosa Cells
Cell Culture
RNA
DNA
Protein Analysis
Cell Freezing
Cell Thawing
Alpha MEM
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