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用三维定量相位成像和机器学习技术对淋巴细胞亚型进行无标签鉴定
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
DOI:

08:58 min

November 19, 2018

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Chapters

  • 00:04Title
  • 01:173D Quantitative Phase Imaging
  • 04:34Quantitative Morphological and Biochemical Feature Extraction and Supervised Learning and Identification
  • 06:42Results: Representative Label-Free Lymphocyte Subtype 3D Phase Imaging Identification
  • 07:56Conclusion

Summary

Automatic Translation

我们描述了一种使用定量相位成像和机器学习算法对淋巴细胞亚型进行无标签识别的协议。对淋巴细胞三维折射率图的测量为单个细胞提供了三维形态和生化信息, 然后用机器学习算法进行分析, 以识别细胞类型。

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