This content is Free Access.
1Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer,Imperial College London, 2Royal British Legion Centre for Blast Injury Studies, Department of Bioengineering,Imperial College London, 3Department of Bioengineering,Imperial College London, 4Department of Life Sciences,Imperial College London, 5Department of Anaesthetics,Royal Berkshire Hospital NHS Foundation Trust, 6Royal Centre for Defence Medicine,Medical Directorate Joint Force Command
Chapters
- 00:04Title
- 00:38Preparation of Hippocampal Organotypic Slices for the Experimental Blast TBI Protocol
- 02:39Submersion and Transport of Tissue Culture Inserts with Hippocampal Organotypic Slices
- 03:44Preparation of the Shock Tube and Hippocampal Organotypic Slice Shock Wave Exposure
- 07:05Results: Typical Shockwave and Resulting Injury in Organotypic Hippocampal Slice
- 08:11Conclusion
Cet article décrit un nouveau modèle de traumatisme crânien de blast primaire. Un tube de choc conduit d’air comprimé est utilisé pour exposer en vitro cultures tranche hippocampe de souris à une seule onde de choc. Il s’agit d’un protocole simple et rapid, générant une lésion tissulaire de cerveau reproductible avec un débit élevé.
