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1Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer,Imperial College London, 2Royal British Legion Centre for Blast Injury Studies, Department of Bioengineering,Imperial College London, 3Department of Bioengineering,Imperial College London, 4Department of Life Sciences,Imperial College London, 5Department of Anaesthetics,Royal Berkshire Hospital NHS Foundation Trust, 6Royal Centre for Defence Medicine,Medical Directorate Joint Force Command
Chapters
- 00:04Title
- 00:38Preparation of Hippocampal Organotypic Slices for the Experimental Blast TBI Protocol
- 02:39Submersion and Transport of Tissue Culture Inserts with Hippocampal Organotypic Slices
- 03:44Preparation of the Shock Tube and Hippocampal Organotypic Slice Shock Wave Exposure
- 07:05Results: Typical Shockwave and Resulting Injury in Organotypic Hippocampal Slice
- 08:11Conclusion
Este artigo descreve um novo modelo de traumatismo crânio-encefálico explosão primária. Um tubo de choque conduzido de ar comprimido é usado para expor em vitro culturas hippocampal fatia de rato a uma única onda de choque. Este é um protocolo simples e rápido, gerando uma lesão de tecidos do cérebro podem ser reproduzidos com um alto throughput.
