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Bruk ferdigmontert plast Microfluidic sjetonger for Compartmentalizing primære Murine nerveceller
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
DOI:

10:50 min

November 02, 2018

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Chapters

  • 00:04Title
  • 01:27Preparation and Coating of the Multicompartment Chips
  • 03:57Seeding Neurons into the Multicompartment Chips
  • 05:03Fluidic Isolation of the Axonal Compartment within the Chip
  • 05:49Performing Axotomy Within the Chip
  • 06:36Fluorescence Immunostaining within the Chip
  • 09:03Results: Neuronal Growth Comparison within Multicompartment PDMS Devices and Plastic Chips
  • 10:21Conclusion

Summary

Automatic Translation

Denne protokollen beskriver bruken av plast sjetonger for kultur og fordeler primære murine neurons. Disse brikkene er prefabrikkerte, brukervennlig og kompatibel med høy oppløsning, lever, og fluorescens bildebehandling. Denne protokollen beskriver hvordan plate rotte hippocampus nerveceller i disse brikkene og utføre fluidic isolasjon, axotomy og immunostai.

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