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Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer’s Patches
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Cancer Research
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JoVE Journal Cancer Research
Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer’s Patches

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer’s Patches

DOI:
10.3791/58490-v

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08:25 min

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November 21, 2018

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, , , ,
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center,Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center,Harvard Medical School

Chapters

  • 00:04Title
  • 00:40Peyer’s Patch (PP) Harvest and Single Cell Suspension Preparation
  • 04:08Surface Marker and Intracellular Protein Staining
  • 06:23Results: Representative Isolated PP Lymphocyte Population Analyses
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, we present a novel and effective protocol for the isolation of lymphocytes from Peyer's Patches (PPs), which can be subsequently used for in vivo and in vitro functional assays as well as flow cytometric studies of follicular T helper and germinal center B cells.

Tags

Keywords: Lymphocyte IsolationPeyer’s PatchesMurineT CellsB CellsFollicular T Helper CellsGerminal Center B CellsCell Isolation Protocol

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