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从小鼠的补丁中提取体液免疫的关键参与者: 先进和优化的淋巴细胞分离协议

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Cancer Research

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JoVE Journal Cancer Research

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer’s Patches

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从小鼠的补丁中提取体液免疫的关键参与者: 先进和优化的淋巴细胞分离协议

DOI:

10.3791/58490-v

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08:25 min

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November 21, 2018

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Yavuz F. Yazicioglu², Halil I. Aksoylar², Rinku Pal², Nikolaos Patsoukis², Vassiliki A. Boussiotis²

¹Division of Hematology-Oncology, Beth Israel Deaconess Medical Center,Harvard Medical School, ²Department of Medicine, Beth Israel Deaconess Medical Center,Harvard Medical School

Chapters

00:04Title
00:40Peyer’s Patch (PP) Harvest and Single Cell Suspension Preparation
04:08Surface Marker and Intracellular Protein Staining
06:23Results: Representative Isolated PP Lymphocyte Population Analyses
08:04Conclusion

Summary

Automatic Translation

在这项研究中, 我们提出了一个新的和有效的方案, 从 peyer 的补丁 (pp) 的淋巴细胞分离, 随后可用于体内和体外功能分析, 以及流式细胞仪研究的卵泡 t辅助和萌发中心 b 细胞。

Tags

Lymphocyte Isolation Peyer’s Patches Murine T Cells B Cells Follicular T Helper Cells Germinal Center B Cells Cell Isolation Protocol

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