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時空ゲスト活性型タグとしてケージの分子接着剤を用いた生細胞内の核内移行を制御
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
DOI:

10:10 min

January 17, 2019

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Chapters

  • 00:04Title
  • 00:29Preparation of Guests with CagedGlue-R Tags
  • 02:17Preparation of Hep3B Cell Sample for Microscopic Observations
  • 02:59Observation of Nuclear Translocation of Small-molecule Guests Triggered by UV Light
  • 04:53Observation of Nuclear Translocation of Small-molecule Guests Triggered by Two-photon NIR Light
  • 05:48Observation of Nuclear Translocation of Macromolecular Guests Triggered by UV Light
  • 07:04Cell Viability Assay
  • 08:20Results: Endosomal Escape and Nuclear Translocation of Guests in Living Cells
  • 09:51Conclusion

Summary

Automatic Translation

このプロトコルでは、ケージ分子接着剤タグを使用して生きている細胞での核内移行を光トリガーについて説明します。このメソッドは、サイト選択的核ターゲット ドラッグデリバリーの有望です。

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