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Biochemistry

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JoVE Journal Biochemistry
Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
 

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Article DOI: 10.3791/59037-v • 11:11 min • February 21st, 2019
February 21st, 2019 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

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Summary
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The objective of this protocol is to label, enrich, and identify substrates of protein kinase CK2 from a complex biological sample such as a cell lysate or tissue homogenate. This method leverages unique aspects of CK2 biology for this purpose.

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Keywords: CK2 Kinase Substrates Biochemical Approach Endogenous Substrates Cell Lysate Tissue Lysate GTPgammaS PNBM Sephadex G-25 Affinity Purification
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