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利用多功能生化方法鉴定新型 ck2 激酶基材
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
DOI:

11:11 min

February 21, 2019

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Chapters

  • 00:04Title
  • 00:35Preparation of the Tissue Samples
  • 01:20Kinase Assay: Thiophosphorylation and Alkylation
  • 02:34Preparation of the Desalting Columns
  • 03:40Removal of PNBM
  • 04:42Immunoprecipitation: Part I
  • 05:32Protein A/G Agarose Bead Preparation
  • 06:39Immunoprecipitation: Part II
  • 08:19Analysis/Validation of Results
  • 09:06Results: Analysis and Identification of Novel CK2 Kinase Substrates
  • 10:48Conclusion

Summary

Automatic Translation

该协议的目的是标记、丰富和识别复杂生物样品 (如细胞裂解物或组织均质) 中的蛋白激酶 ck2 底物。此方法利用 ck2 生物学的独特方面来实现这一目的。

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