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Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
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Chapters
- 00:04Title
- 00:56Murine Hindlimb Dissection and Handling
- 02:36Murine Hindlimb Bone Marrow Isolation
- 03:35Generation of Bone Marrow Macrophages (BMMs)
- 05:17Generation of Osteoclasts from BMMs as Osteoclast Precursors
- 06:09Results: Effect of Anti-c-fms Antibody on Osteoclasts
- 07:35Conclusion
This protocol includes dissection of murine long bones and bone marrow isolation, to generate bone marrow macrophages and produce osteoclasts using macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor Kappa-B ligand (RANKL) or tumor necrosis factor alpha (TNF-α) and their arrest by anti-c-fms antibody, M-CSF receptor.
