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Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations
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Bioengineering
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JoVE Journal Bioengineering
Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

DOI:
10.3791/59214-v

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05:22 min

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March 21, 2019

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, ,
1NanoSquare Research Institute,Osaka Prefecture University, 2Department of Bioscience and Informatics,Osaka Prefecture University, 3Department of Biological Functions Engineering,Kyushu Institute of Technology

Chapters

  • 00:04Title
  • 00:26Hybrid Gel Cube (HGC) Device Fabrication
  • 01:14Hydrogel Initial Cell Cluster Shape Management
  • 02:57Multi-Directional Imaging
  • 04:09Results: Representative Multi-Dimensional Scanning of HGC-Cultured Cells
  • 04:50Conclusion

Summary

Automatic Translation

Automatic Translation

We present a procedure for controlling the initial cell cluster shape in a 3D extracellular matrix to obtain a repeatable pattern formation. A cubic device containing two different hydrogels is employed to achieve multi-directional imaging for tissue pattern formation.

Tags

3D Cell ClusterHybrid Gel CubeRepeatable PatternPolycarbonate Cubic FrameAgaroseExtracellular MatrixCell CultureMicro MoldCell SuspensionCell Culture Medium24-well Plate

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