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用于量化 iPSC 衍生内皮孕激素的血管生成潜力的体外 3D 模型和计算管道

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Developmental Biology

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JoVE Journal Developmental Biology

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Please note that all translations are automatically generated. Click here for the English version.

用于量化 iPSC 衍生内皮孕激素的血管生成潜力的体外 3D 模型和计算管道

DOI:

10.3791/59342-v

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06:36 min

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May 13, 2019

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Cody O. Crosby, Janet Zoldan

¹Department of Biomedical Engineering,University of Texas at Austin

Chapters

00:04Title
00:32Fluorescence-Activated Cell Sorting (FACS) of Endothelial Progenitors
02:08Induced Pluripotent Stem Cell-Endothelial Progenitor (iPSC-EP)-Laden Collagen Hydrogel Encapsulation and Long-Term Culture
03:29EP-Based Vascular Network Visualization
05:22Results: Representative iPSC-EP Vascular Network Generation and Analysis
06:06Conclusion

Summary

Automatic Translation

来自诱导多能干细胞(iPSC-EP)的内皮祖子有可能彻底改变心血管疾病的治疗,并能够创建更忠实的心血管疾病模型。本文介绍了iPSC-EP在三维(3D)胶原蛋白微环境中的封装,并对这些细胞的血管生成电位进行了定量分析。

Tags

Keywords: 3D Model Vasculogenesis IPSC-derived Endothelial Progenitors Computational Pipeline Cardiovascular Disease Therapy Cell Sorting Collagen Hydrogel Encapsulation Cell Culture FACS ROCK Inhibitor

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