/

/

单分子Fürster共振能量传递方法，用于GEN1对霍利迪结分辨率进行实时调查

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Please note that all translations are automatically generated. Click here for the English version.

单分子Fürster共振能量传递方法，用于GEN1对霍利迪结分辨率进行实时调查

DOI:

10.3791/60045-v

•

11:27 min

•

September 18, 2019

•

Mohamed A. Sobhy*¹, Amer Bralić*¹, Vlad-Stefan Raducanu, Muhammad Tehseen, Yujing Ouyang, Masateru Takahashi, Fahad Rashid, Manal S. Zaher, Samir M. Hamdan

¹Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)

Chapters

00:04Title
01:22Flow Cell Preparation
03:01Single-Molecule Förster Resonance Energy Transfer (smFRET) Experiments
06:25Sm-FRET Data Analysis
07:10Two-Color Alternating Excitation (ALEX) FRET Experiment and Analysis
08:24Results: Representative Real-Time HJ Resolution Investigation
10:13Conclusion

Summary

Automatic Translation

这里介绍的是一个执行单分子Fürster共振能量转移以研究HJ分辨率的协议。双色交替激励用于确定解离常数。然后，在实时裂解测定中应用单色时移smFRET，以获得HJ分辨率前的沉思时间分布。

Tags

Single-molecule FÃ¶rster Resonance Energy Transfer (FRET)Holliday Junction GEN1 Real-time Investigation Enzymatic Systems Single-molecule Methods Biomolecular Interactions Optical And Force Measurements Single-channel Flow Cell Quartz Slide Double Adhesive Sheet Polytetrafluoroethylene Tweezers Polyethylene Tubing Epoxy Glue Avidin PBS

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

接合交配测定在接合介入转移蛋白的序列特异性分析

使用定量实时PCR检测大鼠腹膜中的微小RNA表达

激光局部补骨脂素加合物的单分子分析

高分辨率荧光原位杂交在果蝇胚胎和组织中使用 Tyramide 信号放大

转录因子结合、转录、翻译和营业额的实时分析在细胞激活过程中显示全球事件

单分子荧光原位杂交 (smFISH) 在发芽酵母营养生长和减数分裂中的分析

通过高分辨率熔融在水稻种群中识别突变

转化、基因组编辑和拟合氮的热带大麻树帕拉斯波尼亚安德森

CRISPR/Cas9核糖核酸蛋白在急性骨髓性白血病细胞中的RUNX1电子消光器的转录作用研究

有效转录控制质粒表达系统，用于研究原发性奥子拉皮细胞中mRNA笔录稳定性

Read Article