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Solid Phase 11C-Methylation, Purification and Formulation for the Production of PET Tracers
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Chemistry
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JoVE Journal Chemistry
Solid Phase 11C-Methylation, Purification and Formulation for the Production of PET Tracers

Solid Phase 11C-Methylation, Purification and Formulation for the Production of PET Tracers

DOI:
10.3791/60237-v

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09:25 min

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October 24, 2019

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, , , , ,
1McConnell Brain Imaging Centre, Montreal Neurological Institute,McGill University, 2Department of Neurology and Neurosurgery,McGill University

Chapters

  • 00:05Title
  • 01:04Preparation of Buffers and Eluents
  • 02:10Application of the Precursor to the Cartridge
  • 03:19Setting up the Manifold for Automated Synthesis
  • 04:13Radiosynthesis of [11C]PiB
  • 06:46Quality Control Procedures
  • 07:35Results: Radiosynthesis of [11C]PiB
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

We report an efficient carbon-11 radiolabeling technique to produce clinically relevant tracers for Positron Emission Tomography (PET) using solid phase extraction cartridges. 11C-methylating agent is passed through a cartridge preloaded with precursor and successive elution with aqueous ethanol provides chemically and radiochemically pure PET tracers in high radiochemical yields.

Tags

11C-methylationSolid Phase ExtractionPET TracerRadiochemistryAlzheimer’s DiseaseMGlu5 ReceptorAcetate BufferEthanol SolutionTC18 CartridgePrecursorAutomationGMP

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