Journal
/
/
恒流中原发性人类内皮细胞的肺炎球菌感染
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
DOI:

09:34 min

October 31, 2019

, , ,

Chapters

  • 00:04Title
  • 01:29Endothelial Cell Cultivation of HUVEC, Induction of VWF (Von Willebrand Factor)-release and Visualization of Multimerized VWF Strings
  • 05:53Microscopic Evaluation of Bacterial Attachment to VWF-strings in Flow in Real Time and after Sample Fixation
  • 07:12Results: Analyses of Bacterial Attachment to VWF Strings
  • 08:33Conclusion

Summary

Automatic Translation

本研究描述了在定义的流动条件下,在剪切应力下在分化人类原发内皮细胞表面产生的对冯·威勒布兰德因子字符串的肺炎球菌依从的微观监测。通过应用微分免疫染色程序,此协议可扩展到特定细胞结构的详细可视化和细菌定量。

Related Videos

Read Article