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脊柱植入物感染的体内小鼠模型
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JoVE Journal Medicine
In Vivo Mouse Model of Spinal Implant Infection
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脊柱植入物感染的体内小鼠模型

DOI:
10.3791/60560-v

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08:03 min

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June 23, 2020

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, , , , , , , , , , , , , , , , , , , ,
1Department of Orthopaedic Surgery,University of California Los Angeles, 2David Geffen School of Medicine,University of California Los Angeles, 3University of South Carolina School of Medicine,University of South Carolina

Chapters

  • 00:04Introduction
  • 00:39S. aureus Preparation
  • 01:57Mouse Implant Placement and Inoculation
  • 04:09Longitudinal In Vivo Bioluminescence Imaging (BLI)
  • 04:46Implant‐Adherent and Surrounding Tissue Bacteria Quantification
  • 06:16Results: Representative Bacterial Burden Quantification and Analysis
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种新型的脊柱植入物感染体内小鼠模型，其中不锈钢 k 线植入物感染生物发光 金黄色葡萄球 菌 Xen36。通过生物发光成像纵向监测细菌负荷，并在安乐死后通过菌落形成单位计数确认。

Tags

Keywords: Spinal Implant InfectionMouse ModelS. AureusIn VivoBacterial BurdenPostoperative InfectionSpinal SurgeryHost FactorsImplant FactorsTherapeutic FactorsLongitudinal Study

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