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In vivo musemodel af rygmarvsimplantatinfektion
JoVE Journal
Medicine
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JoVE Journal Medicine
In Vivo Mouse Model of Spinal Implant Infection
DOI:

08:03 min

June 23, 2020

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Chapters

  • 00:04Introduction
  • 00:39S. aureus Preparation
  • 01:57Mouse Implant Placement and Inoculation
  • 04:09Longitudinal In Vivo Bioluminescence Imaging (BLI)
  • 04:46Implant‐Adherent and Surrounding Tissue Bacteria Quantification
  • 06:16Results: Representative Bacterial Burden Quantification and Analysis
  • 07:30Conclusion

Summary

Automatic Translation

Protokollen beskriver en ny in vivo musemodel af spinalimplantatinfektion, hvor et rustfrit stål k-wire implantat er inficeret med bioluminescerende Staphylococcus aureus Xen36. Bakteriebyrden overvåges i længderetningen med bioluminescerende billeddannelse og bekræftes med kolonidannende enhedstællinger efter eutanasi.

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