JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
非人类灵长类动物胰腺胰岛耗氧量分析
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption
Please note that all translations are automatically generated.
Click here for the English version.
非人类灵长类动物胰腺胰岛耗氧量分析
DOI:
10.3791/60696-v
•
07:12 min
•
December 18, 2019
•
Joseph M. Elsakr
,
Charles Deeter
,
Valerie Ricciardi
,
Maureen Gannon
4,5,6
1
Department of Molecular Physiology and Biophysics
,
Vanderbilt University
,
2
Agilent Technologies
,
3
Department of Biological Sciences
,
Vanderbilt University
,
4
Department of Veterans Affairs Tennessee Valley
,
5
Department of Medicine
,
Vanderbilt University Medical Center
,
6
Department of Cell and Developmental Biology
,
Vanderbilt University
Chapters
00:05
Title
01:12
Spheroid Microplate Preparation
02:03
Sensor Cartridge Hydration
02:36
Sensor Cartridge Preparation
03:00
Pancreatic Islet Transfer
04:19
Oxygen Consumption Assay
05:06
Results: Representative Pancreatic Islet Oxygen Consumption Analyses
06:09
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该方案证明了对非人类灵长类胰腺胰岛中耗氧量的准确和可重复的测量。微孔板的压网加载技术和涂层为高效测量其他类型的培养球体中的呼吸提供了一个框架。
Tags
Non-human Primate
Pancreatic Islet
Oxygen Consumption
Mitochondrial Function
Islet Transplantation
Diabetes
Cell Adhesive
Sensor Cartridge
Calibrant
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Hemogenic内皮细胞直接诱导和血液中转录因子在人多能干细胞表达
多能高效分化干细胞NKX6-1<sup> +</sup>胰腺祖
Solea Senegalensis
精子中活性氧的细胞内定位评价
斑马鱼视网膜蛋白定位的相关超分辨和电镜观察
无支架三维胰岛素表达 Pancreatoids 的
实验研究体外培养
的小鼠胰祖细胞
高效生成粘附培养中 Hpsc 的胰腺-二叶多布蛋白 1
+
后后福格内胰腺祖细胞
基因型衰老过程中正常人成纤维细胞活性氧和衰老相关分泌表型的定量测定
小鼠胰腺内分泌细胞的单细胞 Transcriptomic 分析
附睾蛋白的合成与分泌分析
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
Read Article