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通过微分试穿素生成马赛克乳腺有机体
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Generation of Mosaic Mammary Organoids by Differential Trypsinization
Please note that all translations are automatically generated.
Click here for the English version.
通过微分试穿素生成马赛克乳腺有机体
DOI:
10.3791/60742-v
•
09:40 min
•
March 11, 2020
•
Stefany Rubio
,
Oscar Cazares
,
Hector Macias
,
Lindsay Hinck
1
Department of Molecular, Cell and Developmental Biology
,
University of California, Santa Cruz
Chapters
00:04
Introduction
00:38
Mammary Gland Digestion
01:59
Mammary Epithelial Tissue Fragment Isolation
03:17
Differential Trypsinization
04:47
Cell Fraction Combination and Extracellular Matrix Embedding
06:06
Organoid Fixation and Immunostaining
07:46
Results: Representative 3D Organoid Culture
08:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
乳腺是一种双层结构,包括外层皮和内光上皮细胞。提出是一种使用微分锥形制备器官的协议。这种有效的方法允许研究人员分别操作这两种细胞类型,以探索有关它们在乳腺形式和功能中的作用的问题。
Tags
Mosaic Mammary Organoids
Differential Trypsinization
Cell Separation
3D Cell Culture
Mammary Gland
Cell Filtration
Cell Culture
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