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用于大型精子细胞三维共焦分析的全山 嗜血杆菌 睾丸免疫染色

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JoVE Journal Biology

Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

Please note that all translations are automatically generated. Click here for the English version.

用于大型精子细胞三维共焦分析的全山嗜血杆菌睾丸免疫染色

DOI:

10.3791/61061-v

•

08:37 min

•

August 27, 2020

•

Natacha Raich, Vincent Contremoulins, Roger E. Karess

¹CNRS, Institut Jacques Monod,Université de Paris, F 75006, Paris, France

Chapters

00:05Introduction
00:47Drosophila Testes Collection
02:14Paraformaldehyde Fixation
03:11Antibody Staining
06:11Results: Representative Whole-Mount Drosophila Testis Immunostaining and 3D Confocal Analysis
07:54Conclusion

Summary

Automatic Translation

我们在这里提出了一个改进的协议，为全安装准备和免疫染色的 嗜血杆菌 睾丸，适合共焦显微镜，也允许可重复和可靠的标签。我们通过对大型S4-S5精子细胞的同位素化实验进行3D表示和量化来说明此协议。

Tags

Keywords: Whole-mount Immunostaining Drosophila Testes 3D Confocal Analysis Spermatocytes NP40 Heptane Fixation Protocol Antibody Penetration Quantifiable Labeling Diploid Germ Cell Evaluation Ringer’s Buffer Paraformaldehyde PBS-T Permeabilization Blocking 3D Imaging

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