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In Vivo Targeting of Xenografted Human Cancer Cells with Functionalized Fluorescent Silica Nanoparticles in Zebrafish
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1Departments of Pharmacology and Medicine, Section of Hematology and Medical Oncology, The Cancer Research Center,Boston University School of Medicine, 2Department of Biomedical Engineering,Tufts University, 3Department of Mechanical Engineering,Tufts University, 4Department of Physics,Tufts University
Chapters
- 00:04Introduction
- 00:54Generation of Casper Zebrafish Embryos
- 02:33Preparing Materials for Cancer Cell Transplantation
- 03:34Preparing Cancer Cells for Transplantation
- 05:20Transplantation of Cancer Cells
- 06:54Injection of Nanoparticles or Vehicle
- 07:40Imaging and Tracking of Nanoparticles and Cancer Cells
- 08:45Results: Colocalization of Cancer Cells and Nanoparticles
- 09:47Conclusion
Described here is a method for utilizing zebrafish embryos to study the ability of functionalized nanoparticles to target human cancer cells in vivo. This method allows for the evaluation and selection of optimal nanoparticles for future testing in large animals and in clinical trials.
