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In Vivo Targeting of Xenografted Human Cancer Cells with Functionalized Fluorescent Silica Nanoparticles in Zebrafish
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1Departments of Pharmacology and Medicine, Section of Hematology and Medical Oncology, The Cancer Research Center,Boston University School of Medicine, 2Department of Biomedical Engineering,Tufts University, 3Department of Mechanical Engineering,Tufts University, 4Department of Physics,Tufts University
Chapters
- 00:04Introduction
- 00:54Generation of Casper Zebrafish Embryos
- 02:33Preparing Materials for Cancer Cell Transplantation
- 03:34Preparing Cancer Cells for Transplantation
- 05:20Transplantation of Cancer Cells
- 06:54Injection of Nanoparticles or Vehicle
- 07:40Imaging and Tracking of Nanoparticles and Cancer Cells
- 08:45Results: Colocalization of Cancer Cells and Nanoparticles
- 09:47Conclusion
Descrito aquí es un método para utilizar embriones de pez cebra para estudiar la capacidad de las nanopartículas funcionalizadas para atacar las células cancerosas humanas in vivo. Este método permite la evaluación y selección de nanopartículas óptimas para futuras pruebas en animales grandes y en ensayos clínicos.
