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Journal
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Biochemistry
/
ラット膵臓組織の迅速かつ費用対効果の高いRNA抽出
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
Please note that all translations are automatically generated.
Click here for the English version.
ラット膵臓組織の迅速かつ費用対効果の高いRNA抽出
DOI:
10.3791/61255-v
•
10:12 min
•
September 19, 2020
•
Sanaz Dastghaib
2
,
Zahra Shahsavar
,
Zahra Karimian
,
Pooneh Mokarram
5
1
Department of Biochemistry, School of Medicine
,
Shiraz University of Medical Sciences
,
2
Endocrinology and Metabolism Research Center, Nemazee Hospital
,
Shiraz University of Medical Sciences
,
3
English Language Department, Faculty of Paramedical Sciences
,
Shiraz University of Medical Sciences
,
4
Department of e-Learning in Medical Sciences, Virtual School and Center of Excellence in e-Learning
,
Shiraz University of Medical Sciences
,
5
Autophagy Research Center, School of Medicine
,
Shiraz University of Medical Sciences
Chapters
00:00
Introduction
00:58
The Removal of the Rat Pancreatic Tissue
03:33
RNA Extraction
08:00
Evaluating RNA Integrity with Denaturation Electrophoresis
08:40
Representative Results: The Evaluation of the RNA Integrity
09:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
単離されたRNAの純度と完全性は、RNA依存アッセイにおいて重要なステップです。ここでは、少量の損傷を受けていない膵臓組織からRNAを抽出する実用的で迅速かつ安価な方法を提示する。
Tags
RNA Extraction
Rapid Method
Cost-effective
Autolysis
RNA Stabilization
RNase Inactivation
RNA Integrity
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