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Fordøjelse af hele museøjne til multiparameterflow cytometrisk analyse af mononukleare fagocytter
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Immunology and Infection
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JoVE Journal Immunology and Infection
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Fordøjelse af hele museøjne til multiparameterflow cytometrisk analyse af mononukleare fagocytter

DOI:
10.3791/61348-v

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09:58 min

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June 17, 2020

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, ,
1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Chapters

  • 00:05Introduction
  • 01:13Digestion of Ocular Tissue
  • 03:34Preparation of Cell Suspension
  • 05:19Staining of Cell Suspension for Mononuclear Phagocytes
  • 06:49Results: Mononuclear Phagocyte Populations in the Mouse Eye
  • 09:26Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol giver en metode til at fordøje hele øjne i en enkelt celle suspension med henblik på multi-parameter flow cytometrisk analyse med henblik på at identificere specifikke okulære mononukleare phagocytiske populationer, herunder monocytter, mikroglia, makrofager, og dendritiske celler.

Tags

DigestionMouse EyesMononuclear PhagocytesFlow CytometryMacrophagesMonocytesMicrogliaDendritic CellsCell Surface MarkersChoroidal NeovascularizationDiabetic RetinopathyExperimental Autoimmune UveitisLupusCytometer ConfigurationDissectionMechanical Disruption

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