Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Methylation Specific Multiplex Droplet PCR using…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering
Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
 

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Article DOI: 10.3791/61421-v • 09:05 min • June 29th, 2020
June 29th, 2020 •
1, 1, 2, 1, 1, 1, 1, 1, 1, 1
1Life Sciences Division, National Research Council of Canada, 2Galenvs Sciences, Inc.

Chapters

Summary
Automatic Translation

Epigenetic markers are used for white blood cell (WBC) subtyping through the quantification of DNA methylation patterns. This protocol presents a multiplex droplet polymerase chain reaction (mdPCR) method using a thermoplastic elastomer (TPE)-based microfluidic device for droplet generation allowing for precise and multiplex methylation-specific target quantification of WBC differential counts.

Tags

Keywords: Methylation-specific Multiplex Droplet PCR Polymer Droplet Generator Hematological Diagnostics Differential Leukocyte Counting Epigenetic Methylation Markers Immunofluorescent Staining Peripheral Blood Mononuclear Cells Bisulfite Conversion Ion Chromatography Column
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter