A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Cardiovascular Research Institute for Intractable Disease, College of Medicine,The Catholic University of Korea, 2Division of Cardiology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, 3Division of Cardiology, Department of Internal Medicine, Uijeongbu St. Mary’s Hospital, College of Medicine,The Catholic University of Korea
Chapters
- 00:05Introduction
- 01:49Preparation of MSCs and Injectable Gelatin Hydrogels
- 03:38In Situ MSC-loading and Three-dimensional in Vitro Culture
- 04:29Induction of Myocardial Infarction in Mice
- 05:44Intramyocardial Transplantation of MSC-loading GH Hydrogels
- 06:37Results: Improving Stem Cell Retention and Engraftment with Injectable Hydrogels
- 08:31Conclusion
La thérapie à base de cellules souches est apparue comme une stratégie efficace pour réparer les tissus cardiaques blessés après l’infarctus du myocarde. Nous fournissons une application in vivo optimale pour la transplantation de cellules souches utilisant des hydrogels de gélatine qui peuvent être enzymatiquement liés.
Tags
Keywords:
Mesenchymal Stem CellsMSCsInjectable HydrogelsIntramyocardial TransplantationMyocardial InfarctionMurine ModelCell RetentionCell SurvivalGrowth FactorsGenetic MaterialsDrugsIn Vitro ExperimentIn Vivo ExperimentCell CultureCell SuspensionTrypsinTrypan BlueCell CountingGH Conjugate SolutionHydrogen PeroxideDual Syringe
