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マウスモデルにおける心筋梗塞後のMSC負荷注入性ヒドロゲルの筋内移植
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Medicine
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JoVE Journal
Medicine
Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model
Please note that all translations are automatically generated.
Click here for the English version.
マウスモデルにおける心筋梗塞後のMSC負荷注入性ヒドロゲルの筋内移植
DOI:
10.3791/61752-v
•
09:19 min
•
September 20, 2020
•
Chan Woo Kim
2
,
Chan Joon Kim
3
,
Eun-Hye Park
2
,
Eunmi Lee
2
,
Eunhwa Seong
2
,
Kiyuk Chang
2
1
Cardiovascular Research Institute for Intractable Disease, College of Medicine
,
The Catholic University of Korea
,
2
Division of Cardiology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine
,
The Catholic University of Korea
,
3
Division of Cardiology, Department of Internal Medicine, Uijeongbu St. Mary’s Hospital, College of Medicine
,
The Catholic University of Korea
Chapters
00:05
Introduction
01:49
Preparation of MSCs and Injectable Gelatin Hydrogels
03:38
In Situ
MSC-loading and Three-dimensional in Vitro Culture
04:29
Induction of Myocardial Infarction in Mice
05:44
Intramyocardial Transplantation of MSC-loading GH Hydrogels
06:37
Results: Improving Stem Cell Retention and Engraftment with Injectable Hydrogels
08:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
幹細胞ベースの治療は、心筋梗塞後に負傷した心臓組織を修復するための効率的な戦略として浮上している。酵素的に架橋できるゼラチンハイドロゲルを用いた幹細胞移植に最適なインビボアプリケーションを提供します。
Tags
Mesenchymal Stem Cells
MSCs
Injectable Hydrogels
Intramyocardial Transplantation
Myocardial Infarction
Murine Model
Cell Retention
Cell Survival
Growth Factors
Genetic Materials
Drugs
In Vitro Experiment
In Vivo Experiment
Cell Culture
Cell Suspension
Trypsin
Trypan Blue
Cell Counting
GH Conjugate Solution
Hydrogen Peroxide
Dual Syringe
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