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Developmental Biology
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应用小鼠部分遗传类胚胎干细胞作为精子的替代品
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
Please note that all translations are automatically generated.
Click here for the English version.
应用小鼠部分遗传类胚胎干细胞作为精子的替代品
DOI:
10.3791/61999-v
•
08:08 min
•
November 19, 2020
•
Eishi Aizawa
,
Charles-Etienne Dumeau
,
Anton Wutz
1
Institute of Molecular Health Sciences, Swiss Federal Institute of Technology
,
ETH Zurich
Chapters
00:05
Introduction
01:09
Holding and Microinjection Pipette Preparation
02:58
Intracytoplasmic Double Knockout Parthenogenetic Haploid Embryonic Stem Cell (DKO-phaESC) Injection
05:34
Results: Representative Establishment and Application of DKO-phaESC
07:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文旨在证明使用部分遗传性单体胚胎干细胞作为精子的替代品,用于构建半克隆胚胎。
Tags
Keywords: Mouse Parthenogenetic Haploid Embryonic Stem Cells
Sperm Substitute
Genomic Imprinting
Embryo Generation
Gamete Manipulation
Microinjection Pipette Preparation
Intracytoplasmic Injection
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